Figure 3. Skp2 is a target of miR-506. (A) The interaction between miR-506 and the Skp2 3’UTR is shown. Mutation of the putative miR-506–binding site on the Skp2 3’UTR is presented. (B) A luciferase reporter assay was carried out to test the binding of miR-506 to the Skp2 3’-UTR. CTR: Control. WT: wild type; Mt: mutation. (C) Western blotting detection of the protein level of Skp2 and its targets in osteosarcoma cells transfected with miR-506 mimics. (D) Quantitative results of (C). *p < 0.05, compared to control.