Research Paper Volume 13, Issue 4 pp 6055—6065

miR-29b restrains cholangiocarcinoma progression by relieving DNMT3B-mediated repression of CDKN2B expression

Overexpression of miR-29b suppresses proliferation and induces cell cycle arrest and apoptosis in cholangiocarcinoma cells. (A) Top: Representative fluorescence images of untransfected (CON), LV-miR-NC-transfected (NC), and LV-miR-29b-transfected (miR-29b) QBC939 cells (original magnification: 100×). Bottom: Relative miR-29b expression detected by qRT-PCR. (B) MTT assay results showing the time-course effect of miR-29b overexpression on the proliferation of QBC939 cells. (C) Representative images from colony formation assays. (D) Relative colony formation percentage. (E) Cell cycle distribution was subjected by flow cytometry. (F) Quantified histograms display the effect of miR-29b overexpression on cell cycle distribution. (G) Flow cytometry plots illustrating apoptosis in Annexin V/PI-stained QBC939 cells. (H) Quantified histograms display the effect of miR-29b overexpression on the apoptosis of QBC939 cells. The values presented are means ± SD. *PP

Figure 2. Overexpression of miR-29b suppresses proliferation and induces cell cycle arrest and apoptosis in cholangiocarcinoma cells. (A) Top: Representative fluorescence images of untransfected (CON), LV-miR-NC-transfected (NC), and LV-miR-29b-transfected (miR-29b) QBC939 cells (original magnification: 100×). Bottom: Relative miR-29b expression detected by qRT-PCR. (B) MTT assay results showing the time-course effect of miR-29b overexpression on the proliferation of QBC939 cells. (C) Representative images from colony formation assays. (D) Relative colony formation percentage. (E) Cell cycle distribution was subjected by flow cytometry. (F) Quantified histograms display the effect of miR-29b overexpression on cell cycle distribution. (G) Flow cytometry plots illustrating apoptosis in Annexin V/PI-stained QBC939 cells. (H) Quantified histograms display the effect of miR-29b overexpression on the apoptosis of QBC939 cells. The values presented are means ± SD. *P<0.05 and **P<0.01compared to the negative control group, as determined by analysis one-way variance (ANOVA), followed by the repeated measures.