Research Paper Advance Articles

LncRNA LINC01305 promotes cervical cancer progression through KHSRP and exosome-mediated transfer

Exosomal LINC01305 promotes the progression of CC. (A) Expression of LINC01305 in C-33A cells treated with RNase A or RNase A plus Triton X-100. (B) Nanoparticle tracking analysis for the number and size of exosomes. (C) Transmission electron microscopy for the morphology of exosomes. Scale bar = 200 μm. (D) Protein expressions of exosomal surface markers. (E) Expression of LINC01305 of exosomes derived from C-33A cells with overexpression or silencing of LINC01305. (F) Expression of LINC01305 in C-33A cells cocultured with exosomes derived from C-33A cells with overexpression of LINC01305. (G) Protein expressions of p65 and STAT3 in C-33A cells cocultured with exosomes derived from C-33A cells with overexpression of LINC01305. (H, I) Representative image of tumor tissues and xenograft mice treated with exosomes derived from C-33A cells with overexpression of LINC01305. (J) Tumor volume of xenograft mice treated with exosomes derived from C-33A cells with overexpression of LINC01305. * P P P

Figure 5. Exosomal LINC01305 promotes the progression of CC. (A) Expression of LINC01305 in C-33A cells treated with RNase A or RNase A plus Triton X-100. (B) Nanoparticle tracking analysis for the number and size of exosomes. (C) Transmission electron microscopy for the morphology of exosomes. Scale bar = 200 μm. (D) Protein expressions of exosomal surface markers. (E) Expression of LINC01305 of exosomes derived from C-33A cells with overexpression or silencing of LINC01305. (F) Expression of LINC01305 in C-33A cells cocultured with exosomes derived from C-33A cells with overexpression of LINC01305. (G) Protein expressions of p65 and STAT3 in C-33A cells cocultured with exosomes derived from C-33A cells with overexpression of LINC01305. (H, I) Representative image of tumor tissues and xenograft mice treated with exosomes derived from C-33A cells with overexpression of LINC01305. (J) Tumor volume of xenograft mice treated with exosomes derived from C-33A cells with overexpression of LINC01305. * P < 0.05, ** P < 0.01, *** P < 0.001.