Research Paper Volume 13, Issue 5 pp 7180—7189

Neuroprotective effect of hydrogen sulfide against glutamate-induced oxidative stress is mediated via the p53/glutaminase 2 pathway after traumatic brain injury

Treatment with H2S inhibited TBI-induced apoptosis. (A) Western blot was performed to analyze the protein level of Bcl-2, Bax, and Cleaved caspase-3 in TBI-challenged rats after treatment with NaHS. (B–D) The band density of Bcl-2 (B), Bax (C), and Cleaved caspase-3 (D) was analyzed using Image J. (E) The apoptosis rate of cerebral cortex in TBI rats was analyzed by Tunnel staining. * P

Figure 2. Treatment with H2S inhibited TBI-induced apoptosis. (A) Western blot was performed to analyze the protein level of Bcl-2, Bax, and Cleaved caspase-3 in TBI-challenged rats after treatment with NaHS. (BD) The band density of Bcl-2 (B), Bax (C), and Cleaved caspase-3 (D) was analyzed using Image J. (E) The apoptosis rate of cerebral cortex in TBI rats was analyzed by Tunnel staining. * P<0.05, vs. TBI and TBI+ vehicle groups. #P<0.05 vs. sham group.