Figure 7. LncRNA ZFAS1 regulated fibrosis via interacting with miR-4711-5p/wnt4. (A) The biological website predicted the existence of binding sites between lncRNA ZFAS1 and miR-4711-5p. Luciferase assay confirmed the relationship between lncRNA ZFAS1 with miR-4711-5p. (B) Cell morphology detection. (C) RT-PCR assay was performed to measure the expression of Col1a1, Col3a1, MMP2, MMP9, α-SMA, and CTGF. (D) The represented image of immunofluorescence for staining vimentin and α-SMA. (E) The biological website predicted the existence of binding sites between wnt4 and miR-4711-5p. (F) The effect of lncRNA ZFAS1 and miR-4711-5p on the wnt4/β-catenin signal pathway. *P<0.05, **P<0.01 vs. TGF-β1+exo-NC group. #P <0.05 vs. TGF-β1+exo-ZFAS1.