Research Paper Volume 13, Issue 5 pp 7538—7548

SEDT2/METTL14-mediated m6A methylation awakening contributes to hypoxia-induced pulmonary arterial hypertension in mice

The expression of SETD2 mediates H3K36me3 and METTL14 are enhanced in hypoxia-induced PAH, whereas impaired by SMCs specific SETD2 deficient. (A) Represent images of Western blotting analysis. The statistical data showed that hypoxia markedly elevated the protein level of SETD2 (B), H3K36me3 (C) and METTL14 (E) in SMCs, but SMCs specific SETD2 deficient impaired the level of the protein level of H3K36me3 and METTL14. However, the protein level of METTL3 (D) was changed neither by hypoxia nor SMCs specific SETD2 deficient. All data were presented as Mean±SD (n=5). *P

Figure 5. The expression of SETD2 mediates H3K36me3 and METTL14 are enhanced in hypoxia-induced PAH, whereas impaired by SMCs specific SETD2 deficient. (A) Represent images of Western blotting analysis. The statistical data showed that hypoxia markedly elevated the protein level of SETD2 (B), H3K36me3 (C) and METTL14 (E) in SMCs, but SMCs specific SETD2 deficient impaired the level of the protein level of H3K36me3 and METTL14. However, the protein level of METTL3 (D) was changed neither by hypoxia nor SMCs specific SETD2 deficient. All data were presented as Mean±SD (n=5). *P<0.05 vs. corresponding group.