Research Paper Volume 13, Issue 6 pp 8228—8247

Long non-coding RNAs ENST00000429730.1 and MSTRG.93125.4 are associated with metabolic activity in tuberculosis lesions of sputum-negative tuberculosis patients

Validation of lncRNAs and mRNAs by quantitative real-time polymerase chain reaction (qRT-PCR) and prediction efficiencies evaluated by receiver operating characteristic (ROC) curve analysis. (A) expressions changes of lncRNAs and mRNAs that had relatively larger number of degree in lncRNA-target-pathway network detected by qRT-PCR. The red bars indicate the expression changes in PET-high samples comparing with PET-low samples according to the RNA-sequencing data of PET-high (n = 5) and PET-low (n = 5) samples. The green bars indicate the expression changes in PET-high samples (n = 11) comparing with PET-low samples (n = 11) according to the qRT-PCR experiment results. The horizontal axis means the names of lncRNAs or mRNAs, and the vertical axis means the relative expression (log2 FC). *: p B) ROC curve of ENST00000429730.1. (C) ROC curve of MSTRG.93125.4.

Figure 4. Validation of lncRNAs and mRNAs by quantitative real-time polymerase chain reaction (qRT-PCR) and prediction efficiencies evaluated by receiver operating characteristic (ROC) curve analysis. (A) expressions changes of lncRNAs and mRNAs that had relatively larger number of degree in lncRNA-target-pathway network detected by qRT-PCR. The red bars indicate the expression changes in PET-high samples comparing with PET-low samples according to the RNA-sequencing data of PET-high (n = 5) and PET-low (n = 5) samples. The green bars indicate the expression changes in PET-high samples (n = 11) comparing with PET-low samples (n = 11) according to the qRT-PCR experiment results. The horizontal axis means the names of lncRNAs or mRNAs, and the vertical axis means the relative expression (log2 FC). *: p < 0.05, **: p < 0.01, ***: p < 0.001 comparing with PET-low samples based on qRT-PCR experiment results. (B) ROC curve of ENST00000429730.1. (C) ROC curve of MSTRG.93125.4.