Figure 4. Chondrogenesis tended to be reduced in the foot fibroblasts. (A) Representative images of Alcian blue staining after two weeks induction of chondrogenesis in the trunk and foot fibroblasts of WS1. Bar = 300 μm. (B) Quantification of the diameter of chondrogenic spheroids. WS1-trunk, WS2-trunk, and WS1-foot were included. Data are means ± SEM. For statistical analysis, student t-test was performed (*p<0.05). (C) Relative gene expression analyzed by qRT-PCR. Data are means ± SEM of two patients with three technical replicates. For statistical analysis, student t-test was performed (ns, not significant; *p<0.05; **p<0.01).