Research Paper Volume 13, Issue 8 pp 11083—11095

circUBAP2 exacerbates malignant capabilities of NSCLC by targeting KLF4 through miR-3182 modulation

(A, B) CCK8 assay on A549 and NCI-H1299 cell line, which were transfected by sh-circUBAP2

Figure 4. (A, B) CCK8 assay on A549 and NCI-H1299 cell line, which were transfected by sh-circUBAP2_046 or circUBAP2_046 overexpression vector. (C, D) Cell migration and invasion assay on A549 and NCI-H1299 cell transfected with sh-circUBAP2_046 or control. (EG) WB and RT-PCR analysis on cellular proliferation and migration biomarkers including Ki67, E-Cadherin, Fibronectin and MMP9 in A549 /NCI-H1299 cell groups transfected with sh-circUBAP2_046 or circUBAP2_046 overexpression vector. (H, I) Impact of circUBAP2_046 silencing or over-expression on tumor growth and weight from in vivo A549/NCI-H1299 cell-derived xenograft models (5 tumors were measured for each group). (J, K) RT-PCR detection of circUBAP2_046 and UBAP2 mRNA expression level in xenograft tumor tissue of A549/NCI-H1299 cells transfected with sh-circUBAP2_046 or circUBAP2_046 overexpression vector (5 tumors were measured for each group).