Figure 5. (A) Bioinformatic prediction on the binding sites of wild-type circUBAP2_046 or mutated circUBAP2_046 with miR-3182. (B) Correlations of mRNA expression value between miR-3182 and circUBAP2_046 in NSCLC lung cancer tissue samples. (C, D) Dual-luciferase reporter gene assay demonstrated the binding of circUBAP2_046 and miR-3182 in A549 and NCI-H1299 cell line models. (E, F) RNA immunoprecipitation assay demonstrated significantly enriched binding of circUBAP2_046 and miR-3182 with anti-Ago2 antibody compared with control IgG antibody in A549 and NCI-H1299 cell line models. (G) RT-PCR analysis about the modulative effects on miR-3182 expression by circUBAP2_046 silencing or overexpression.