Research Paper Volume 13, Issue 8 pp 11083—11095

Figure 7. (A) Western-Blot and RT-PCR analysis on the siRNA inhibitory effects on the protein and mRNA expression level of KLF4. (B) CCK8 cell viability assay on NCI-H1299 cell line model transfected with circUBAP2_046 over-expression vector with/without KLF4 siRNA. (C–E) Cell survival assay on NCI-H1299 treated with escalated doses of Docetaxel. Cell line model was transfected with circUBAP2_046 over-expression vector with/without KLF4 siRNA. (F, G) Western-Blot and RT-PCR analysis on the KLF4 protein and mRNA expression value in A539 cell line model influenced by transfection of circUBAP2_046 over-expression vector with/without miR-3182 mimic. (H, I) Western-Blot and RT-PCR analysis on the KLF4 protein and mRNA expression value in NCI-H1299 cell line model influenced by transfection of circUBAP2_046 over-expression vector with/without miR-3182 mimic.