Research Paper Volume 13, Issue 7 pp 10603—10618

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma

DCAF15 inhibits epithelial-mesenchymal transition (EMT) of HCC cells. (A–B) Western blot analysis showed the protein level of EMT markers after transfecting with control or DCAF15 overexpression plasmids in HCC cells. (C–D) Western blot analysis showed the protein level of EMT markers after transfecting with control or DCAF15 siRNA in HCC cells. (E) qRCR analysis showed relative mRNA level of EMT markers after transfecting with control or DCAF15 overexpression plasmids in HCC cells. (F) qRCR analysis showed relative mRNA level of EMT markers after transfecting with control or DCAF15 siRNA in HCC cells. (G) Protein level of E-cadherin and vimentin after transfecting with control or DCAF15 overexpression plasmids in HCC cells was visualized by fluorescence microscopy. Scale bars, 100 μm. (H) Protein level of E-cadherin and vimentin after transfecting with control or DCAF15 siRNA in HCC cells was visualized by fluorescence microscopy. Scale bars, 100 μm.

Figure 6. DCAF15 inhibits epithelial-mesenchymal transition (EMT) of HCC cells. (AB) Western blot analysis showed the protein level of EMT markers after transfecting with control or DCAF15 overexpression plasmids in HCC cells. (CD) Western blot analysis showed the protein level of EMT markers after transfecting with control or DCAF15 siRNA in HCC cells. (E) qRCR analysis showed relative mRNA level of EMT markers after transfecting with control or DCAF15 overexpression plasmids in HCC cells. (F) qRCR analysis showed relative mRNA level of EMT markers after transfecting with control or DCAF15 siRNA in HCC cells. (G) Protein level of E-cadherin and vimentin after transfecting with control or DCAF15 overexpression plasmids in HCC cells was visualized by fluorescence microscopy. Scale bars, 100 μm. (H) Protein level of E-cadherin and vimentin after transfecting with control or DCAF15 siRNA in HCC cells was visualized by fluorescence microscopy. Scale bars, 100 μm.