Figure 2. Heat-inactivated Lb. fermentum BGHV110 triggers autophagy in C. elegans. Representative fluorescence images (A) and quantifications (B) of day 1 adult DA2123 transgenic animals expressing GFP::LGG-1 under the lgg-1 promoter after overnight BGHV110 treatment (n=4–10, results are representative of 3 independent assays). Western blots (C) and densitometric analysis (D) showing the levels of GFP::LGG-1 and cleaved GFP proteins isolated from the DA2123 transgenic strain on day 1 of adulthood after overnight BGHV110 treatment. HSC-70 was used as a loading control (n=3, three independent experiments). Expression of hlh-30 and autophagy-related genes was measured by qRT-PCR in L4 stage WT animals after 6 h of treatment with (E) heat-inactivated BGHV110 and (F) heat-inactivated OP50 supplemented with heat-inactivated BGHV110 in a 1:2 ratio (n=3, three independent experiments). All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control (*p < 0.05, **p < 0.01, ***p < 0.001).