Figure 3. Heat-inactivated Lb. fermentum BGHV110 delays aging in C. elegans in an HLH-30-dependent manner. (A) Lifespan curve of the hlh-30 (tm1978) mutant fed with heat-inactivated control (OP50) and BGHV110 bacteria from the L4 developmental stage maintained at 20° C (n=100 per group, results from one of two experiments with similar results are shown). (B) Body bending rate was measured in the hlh-30 (tm1978) mutant on day 10 of adulthood (n=10 per group, results are representative of 3 independent assays). (C) Expression of autophagy-related genes was measured by qRT-PCR in the L4 stage of the hlh-30 (tm1978) mutant after 6 h of treatment with heat-inactivated BGHV110 (n=3, three independent experiments). All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control (*p < 0.05, **p < 0.01). The log-rank (Mantel-Cox) test was used to assess the p-value in lifespan analysis.