Figure 4. Heat-inactivated Lb. fermentum BGHV110 affects lipid metabolism. (A) Oil Red O staining of the WT and hlh-30 mutant animals fed with heat-inactivated control (OP50) and BGHV110 bacteria analyzed on day 5 of adulthood. (B) Dye intensity quantification of the WT and hlh-30 mutant animals compared to OP50 control (n=25 per group, results are representative of 3 independent assays). (C) The expression of lipl-1, 2, 3, 4, 5 genes was measured by qRT-PCR in the L4 stage WT and hlh-30 mutant after 6 h of treatment with heat-inactivated BGHV110 (n=3, three independent experiments). All values are presented as mean ± SD. One-way ANOVA followed by Tukey post hoc test for multiple comparisons was used (*p < 0.05, **p < 0.01, ***p < 0.001).