Research Paper Volume 13, Issue 6 pp 8040—8054

Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy

Heat-inactivated Lb. fermentum BGHV110 effects on the SKN-1 detoxification pathway. GFP fluorescence intensity was visualized by fluorescence microscopy (A, left panel) and intensity was quantified (A, right panel) in day 1 adult NV6 transgenic animals expressing GFP under the gst-4 promoter after overnight BGHV110 treatment (n=10, results are representative of 3 independent assays). (B) Expression of skn-1 and gst-4 genes was measured by qRT-PCR in L4 stage WT animals after 6 h of treatment with BGHV110 (n=3, three independent experiments). (C) Overnight OP50 and BGHV110 treated WT animals were exposed to 20 mM H2O2 on day 1 of adulthood and assayed for survival 4 h later. All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control. The log-rank (Mantel-Cox) test was used to assess the p-value in the H2O2 assay.

Figure 5. Heat-inactivated Lb. fermentum BGHV110 effects on the SKN-1 detoxification pathway. GFP fluorescence intensity was visualized by fluorescence microscopy (A, left panel) and intensity was quantified (A, right panel) in day 1 adult NV6 transgenic animals expressing GFP under the gst-4 promoter after overnight BGHV110 treatment (n=10, results are representative of 3 independent assays). (B) Expression of skn-1 and gst-4 genes was measured by qRT-PCR in L4 stage WT animals after 6 h of treatment with BGHV110 (n=3, three independent experiments). (C) Overnight OP50 and BGHV110 treated WT animals were exposed to 20 mM H2O2 on day 1 of adulthood and assayed for survival 4 h later. All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control. The log-rank (Mantel-Cox) test was used to assess the p-value in the H2O2 assay.