Research Paper Volume 13, Issue 6 pp 8040—8054

Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy

Mitochondrial homeostasis and the UPRmt assessment. (A) Western blots and (B) densitometric analysis showing levels of HSP-60, ATP5A and NDUFS3 proteins isolated from WT animals on day 1 of adulthood after overnight treatment with heat-inactivated BGHV110. HSC-70 was used as a loading control (n=3, three independent experiments). Expression of cox-1, mtss-1 and polg-1 genes was measured by qRT-PCR after (C) 1 day and (D) 5 days of treatment with heat-inactivated BGHV110 (n=3, three independent experiments). All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control (*p

Figure 6. Mitochondrial homeostasis and the UPRmt assessment. (A) Western blots and (B) densitometric analysis showing levels of HSP-60, ATP5A and NDUFS3 proteins isolated from WT animals on day 1 of adulthood after overnight treatment with heat-inactivated BGHV110. HSC-70 was used as a loading control (n=3, three independent experiments). Expression of cox-1, mtss-1 and polg-1 genes was measured by qRT-PCR after (C) 1 day and (D) 5 days of treatment with heat-inactivated BGHV110 (n=3, three independent experiments). All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control (*p < 0.05; **p < 0.01).