Research Paper Volume 13, Issue 10 pp 13859—13875

Curcumin inhibits the proliferation and migration of vascular smooth muscle cells by targeting the chemerin / CMKLR1 / LCN2 axis

Curcumin inhibits CMKLR1 and LCN2 expression. (A) IHC analysis of chemerin / CMKLR1 and LCN2 expressions in aorta tissues of HFD ApoE-/- mice with or without curcumin treatment. Scale bar = 100 μm. Red arrows indicate plaques. (B–D) Chemerin / CMKLR1 and LCN2 expression score analysis data are presented as means ± SDs. n.s., no significant; *, pppE) Western blots showing CMKLR1, MMP-9, LCN2, β-catenin and PCNA expressions in cells treated with curcumin (48 h) for different durations. (F) mRNA expression of CMKLR1 with or without curcumin treatment (48 h) as determined by quantitative real-time PCR.

Figure 5. Curcumin inhibits CMKLR1 and LCN2 expression. (A) IHC analysis of chemerin / CMKLR1 and LCN2 expressions in aorta tissues of HFD ApoE-/- mice with or without curcumin treatment. Scale bar = 100 μm. Red arrows indicate plaques. (BD) Chemerin / CMKLR1 and LCN2 expression score analysis data are presented as means ± SDs. n.s., no significant; *, p< 0.05; **, p< 0.01; ***, p< 0.001. (E) Western blots showing CMKLR1, MMP-9, LCN2, β-catenin and PCNA expressions in cells treated with curcumin (48 h) for different durations. (F) mRNA expression of CMKLR1 with or without curcumin treatment (48 h) as determined by quantitative real-time PCR.