Figure 5. Curcumin inhibits CMKLR1 and LCN2 expression. (A) IHC analysis of chemerin / CMKLR1 and LCN2 expressions in aorta tissues of HFD ApoE-/- mice with or without curcumin treatment. Scale bar = 100 μm. Red arrows indicate plaques. (B–D) Chemerin / CMKLR1 and LCN2 expression score analysis data are presented as means ± SDs. n.s., no significant; *, p< 0.05; **, p< 0.01; ***, p< 0.001. (E) Western blots showing CMKLR1, MMP-9, LCN2, β-catenin and PCNA expressions in cells treated with curcumin (48 h) for different durations. (F) mRNA expression of CMKLR1 with or without curcumin treatment (48 h) as determined by quantitative real-time PCR.