Research Paper Advance Articles

Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1

Successful construction of DEPDC1B knockdown. (A) The expression level of DEPDC1B in SK-HEP-1, Hep3B2.1-7, huh-7 was significantly higher than in HCCLM3 (PB) The fluorescence of cells, which were infected with shCtrl, shDEPDC1B for 72 h, observed by microscope demonstrates a >80% efficiency of infection and the normal cell condition. The results of qRT-PCR show that, after the infection of lentivirus: (C) Compared with the shCtrl group, in HEP3B2.1-7 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 71.35% (PD) compared with shCtrl group, in HEP3B2.1-7 and SK-HEP-1 cells: the protein level of DEPDC1B in shDEPDC1B group was down-regulated. *: P

Figure 2. Successful construction of DEPDC1B knockdown. (A) The expression level of DEPDC1B in SK-HEP-1, Hep3B2.1-7, huh-7 was significantly higher than in HCCLM3 (P<0.05). (B) The fluorescence of cells, which were infected with shCtrl, shDEPDC1B for 72 h, observed by microscope demonstrates a >80% efficiency of infection and the normal cell condition. The results of qRT-PCR show that, after the infection of lentivirus: (C) Compared with the shCtrl group, in HEP3B2.1-7 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 71.35% (P<0.001) and in SK-HEP-1 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 74.1% (P<0.001). The results of Western blot show that, after the infection of lentivirus: (D) compared with shCtrl group, in HEP3B2.1-7 and SK-HEP-1 cells: the protein level of DEPDC1B in shDEPDC1B group was down-regulated. *: P <0.05. **: P <0.01. ***: P <0.001.