Research Paper Volume 13, Issue 16 pp 20094—20115

Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1

Successful construction of DEPDC1B knockdown. (A) The expression level of DEPDC1B in SK-HEP-1, Hep3B2.1-7, huh-7 was significantly higher than in HCCLM3 (PB) The fluorescence of cells, which were infected with shCtrl, shDEPDC1B for 72 h, observed by microscope demonstrates a >80% efficiency of infection and the normal cell condition. The results of qRT-PCR show that, after the infection of lentivirus: (C) Compared with the shCtrl group, in HEP3B2.1-7 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 71.35% (PD) compared with shCtrl group, in HEP3B2.1-7 and SK-HEP-1 cells: the protein level of DEPDC1B in shDEPDC1B group was down-regulated. *: P

Figure 2. Successful construction of DEPDC1B knockdown. (A) The expression level of DEPDC1B in SK-HEP-1, Hep3B2.1-7, huh-7 was significantly higher than in HCCLM3 (P<0.05). (B) The fluorescence of cells, which were infected with shCtrl, shDEPDC1B for 72 h, observed by microscope demonstrates a >80% efficiency of infection and the normal cell condition. The results of qRT-PCR show that, after the infection of lentivirus: (C) Compared with the shCtrl group, in HEP3B2.1-7 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 71.35% (P<0.001) and in SK-HEP-1 cells, the knockdown efficiency of DEPDC1B in shDEPDC1B group was 74.1% (P<0.001). The results of Western blot show that, after the infection of lentivirus: (D) compared with shCtrl group, in HEP3B2.1-7 and SK-HEP-1 cells: the protein level of DEPDC1B in shDEPDC1B group was down-regulated. *: P <0.05. **: P <0.01. ***: P <0.001.