Research Paper Advance Articles

Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1

DEPDC1B knockdown inhibited HCC cell progression in vivo. (A) The results of MTT assay show that, after the infection of lentivirus: compared with shCtrl group, the cells in shDEPDC1B group exhibited slower proliferation rate (PB) The results of flow cytometry demonstrate that, after the infection of lentivirus: compared with shCtrl group, apoptosis percentage was increased in shDEPDC1B group (PC) The results of flow cytometry show that, compared with shCtrl group, in shDEPDC1B group, the percentage of SK-HEP-1 cells in G2 phase increased (PD). (E) The results of wound-healing assay showed that, in HEP3B2.1-7 and SK-HEP-1cells, compared with shCtrl group, the migration rate of cells in shDEPDC1B group was decreased by 68% (P

Figure 3. DEPDC1B knockdown inhibited HCC cell progression in vivo. (A) The results of MTT assay show that, after the infection of lentivirus: compared with shCtrl group, the cells in shDEPDC1B group exhibited slower proliferation rate (P<0.001). (B) The results of flow cytometry demonstrate that, after the infection of lentivirus: compared with shCtrl group, apoptosis percentage was increased in shDEPDC1B group (P<0.001). (C) The results of flow cytometry show that, compared with shCtrl group, in shDEPDC1B group, the percentage of SK-HEP-1 cells in G2 phase increased (P<0.01), and the percentage of Hep3B2.1-7cells in G2 phase increased significantly in shDEPDC1B group (P<0.001). Transwell assay showed that, after the infection of lentivirus: compared with shCtrl group, the migration ability of cells in shDEPDC1B group was inhibited (P<0.001) (D). (E) The results of wound-healing assay showed that, in HEP3B2.1-7 and SK-HEP-1cells, compared with shCtrl group, the migration rate of cells in shDEPDC1B group was decreased by 68% (P<0.001) and 47% (P<0.001). **: P <0.01. ***: P <0.001.