Research Paper Advance Articles

Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1

Functions on cell progression. (A) The results of Celigo cell counting assay show that, compared with NC(OE+KD) group: the cells in DEPDC1B+NC (KD) group exhibited faster proliferation rate (PB) The results of colony formation assay show that, compared to NC(OE+KD) group: the cell colony number in DEPDC1B+NC(KD) group was significantly increased (PC) The results of flow cytometry demonstrate that: compared to NC(OE+KD) group: cell apoptosis was decreased in DEPDC1B+NC(KD) group (P

Figure 6. Functions on cell progression. (A) The results of Celigo cell counting assay show that, compared with NC(OE+KD) group: the cells in DEPDC1B+NC (KD) group exhibited faster proliferation rate (P<0.001), and the cells in shCDK1+NC(OE) group exhibited slower proliferation rate (P<0.001). Compared to DEPDC1B+NC(KD) group, the cells in DEPDC1B+shCDK1 group exhibited slower proliferation rate (P<0.01). The cells in DEPDC1B+shCDK1 group exhibit faster proliferation rate, compared with shCDK1+NC(OE) group (P<0.01). (B) The results of colony formation assay show that, compared to NC(OE+KD) group: the cell colony number in DEPDC1B+NC(KD) group was significantly increased (P<0.001), while the cell colony number in shCDK1+NC(OE) group was significantly decreased (P<0.001). Compared with DEPDC1B+NC(KD) group, cell colony number was significantly decreased in DEPDC1B+shCDK1 group (P<0.001). Cell colony number in DEPDC1B+shCDK1 group was significantly increased, compared to shCDK1+NC(OE) group (P<0.01). (C) The results of flow cytometry demonstrate that: compared to NC(OE+KD) group: cell apoptosis was decreased in DEPDC1B+NC(KD) group (P<0.01), and in shCDK1+NC(OE) group, cell apoptosis was significantly increased (P<0.001). Compared with DEPDC1B+NC(KD) group, cell apoptosis was significantly increased in DEPDC1B+shCDK1 group (P<0.001). Compared to shCDK1+NC(OE) group, cell apoptosis was decreased in DEPDC1B+shCDK1 group (P<0.001). **: P <0.01. ***: P <0.001.