Research Paper Volume 13, Issue 16 pp 20094—20115

Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1

Detection of cell migration ability, and interaction between CDK1 and DEPDC1B. (A) The results of wound-healing assay and Transwell assay showed that, compared to NC(OE+KD) group: the migration rate of cells was significantly increased in DEPDC1B+NC(KD) group (48 h) (PB) The transwell assay shows that, compared to NC(OE+KD) group: the migration ability of cells in DEPDC1B+NC(KD) group was significantly increased (PC, D) The results of co-immunoprecipitation. *: P

Figure 7. Detection of cell migration ability, and interaction between CDK1 and DEPDC1B. (A) The results of wound-healing assay and Transwell assay showed that, compared to NC(OE+KD) group: the migration rate of cells was significantly increased in DEPDC1B+NC(KD) group (48 h) (P<0.001), while in shCDK1+NC(OE) group (48 h), the migration rate of cells was significantly decreased (P<0.001). Compared to DEPDC1B+NC(KD) group, the migration rate of cells was significantly decreased in DEPDC1B+shCDK1 group (48 h) (P<0.001). Compared with shCDK1+NC(OE) group, the migration rate of cells was significantly increased in DEPDC1B+shCDK1 group (48 h) (P<0.05). (B) The transwell assay shows that, compared to NC(OE+KD) group: the migration ability of cells in DEPDC1B+NC(KD) group was significantly increased (P<0.001), and the migration ability of cells in shCDK1+NC(OE) group is significantly decreased (P<0.001). Compared to DEPDC1B+NC(KD) group, the migration ability of cells was significantly decreased in DEPDC1B+shCDK1 group (P<0.001). Compared with shCDK1+NC(OE) group, the migration ability of cells in DEPDC1B+shCDK1 group was significantly increased (P<0.001). (C, D) The results of co-immunoprecipitation. *: P <0.05. **: P <0.01. ***: P <0.001.