Figure 3. miR-340-5p was a direct target of RUSC1-AS1. (A) ENCORI, Starbase and miRDB predicted miRNAs that could bind to RUSC1-AS1. (B) Predicted binding sites of miR-340-5p and RUSC1-AS1. (C) The miR-340-5p expression after mimics-NC and miR-mimics transfection. (D–E) Luciferase reporter assay in U2OS and HOS cells showed direct binding of RUSC1-AS1 and miR-340-5p. (F–G) RIP assay in U2OS and HOS cells showed direct binding of RUSC1-AS1 and miR-340-5p. (H) The miR-340-5p expression after si-RUSC1-AS1 or si-NC transfection. (I) miR-340-5p expression in osteosarcoma tissues and adjacent normal tissues. (J) Linear correlation between miR-340-5p and RUSC1-AS1.