Figure 7. Narciclasine-induced autophagy is associated with JNK activity in cardiomyocytes. Neonatal rat cardiomyocytes were pretreated with narciclasine (300 nmol/L, 30 min) or SP600125 (SP, 20 μmol/L, 30 min) before LPS challenge. (A) JNK activity in the presence or absence of narciclasine was measured by western blotting. (B) The levels of autophagy-associated proteins, p-JNK, and JNK were examined by western blotting. n = 3 per group. The data are shown as the means ± SEM. *P < 0.05 vs. the PBS group. #P < 0.05 vs. the LPS group.