Figure 2.Pressure preconditioning did not affect the cytotoxicity of αROR1-CAR T cells. (A) Pressure preconditioning did not affect αROR1-CAR expression. αROR1-CAR T cells were maintained in a pressurized incubator (+100 mmHg) for 24 hours and the expression of αROR1-CAR was detected by flow cytometry. Shown are data from 2 biological repeats. (B) αROR1-CAR T cells preconditioned in pressurized incubator (+100 mmHg) for 24 hours were co-cultured with non-preconditioned A549-Red-Fluc cells under normal conditions. Viability at indicated time points was measured by luciferase activity and converted to cytotoxicity. (C–E) Cytokines (IL-2, IFN-γ, and TNF-α) in the medium after 16 hours of co-culture shown in (B) were measured by ELISA.
