Research Paper Volume 13, Issue 12 pp 16009—16023

Figure 5. Calycosin suppresses breast cancer cell migration and invasiveness by inhibiting EMT. (A) Western blot analysis shows relative levels of CD147, MMP-2, MMP-9, E-cadherin, N-cadherin, and Vimentin proteins in control and calycosin-treated T47D and MCF-7 breast cancer cells. (B) Western blot analysis shows relative levels of CD147, MMP-2, MMP-9, E-cadherin, N-cadherin, and Vimentin proteins in T47D and MCF-7 breast cancer cells treated with different concentrations of TGFβ1. (C) Representative images of wound healing assay at 0 and 24 h show migration ability of T47D and MCF-7 breast cancer cells treated with calycosin, TGFβ1, or calycosin plus TGFβ1. (D) Transwell invasion assay results show the invasiveness of T47D and MCF-7 breast cancer cells treated with calycosin, TGFβ1, or calycosin plus TGFβ1. The data were represented as means ± SD. *P<0.05; **P<0.01; ***P<0.001.