Research Paper Volume 13, Issue 11 pp 15479—15490

Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a mouse model of allergic asthma

Impairing of SIRT1 exacerbates airway inflammation following allergen exposure.Sirt1fl/fl-LysMCre mice and Sirt1fl/fl littermates were established allergic asthma model as described in the methods section (n = 5-8 for each group). (A) SIRT1 expression in lung tissues of Sirt1fl/fl and Sirt1fl/fl-LysMCre mice were assessed using Western blot; (B, C) Total cells and differential cell counts in BALF were measured; (D) Representative photomicrographs of lung inflammation expression are shown (Scale bar, 100 μm); (E) Semiquantification of inflammation expression in the lungs were preformed using Image Pro 6.1 software; (F) Representative photomicrographs of mucus production are shown (red arrows) (Scale bar, 100 μm); (G) Semiquantification of mucus production in the lungs were preformed using Image Pro 6.1 software. Data are presented as Mean ± SEM of three independent experiments. *P

Figure 4. Impairing of SIRT1 exacerbates airway inflammation following allergen exposure.Sirt1fl/fl-LysMCre mice and Sirt1fl/fl littermates were established allergic asthma model as described in the methods section (n = 5-8 for each group). (A) SIRT1 expression in lung tissues of Sirt1fl/fl and Sirt1fl/fl-LysMCre mice were assessed using Western blot; (B, C) Total cells and differential cell counts in BALF were measured; (D) Representative photomicrographs of lung inflammation expression are shown (Scale bar, 100 μm); (E) Semiquantification of inflammation expression in the lungs were preformed using Image Pro 6.1 software; (F) Representative photomicrographs of mucus production are shown (red arrows) (Scale bar, 100 μm); (G) Semiquantification of mucus production in the lungs were preformed using Image Pro 6.1 software. Data are presented as Mean ± SEM of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001.