Figure 4. HMGA1 -CCDC43 axis promote development and progression. (A) Western blot analysis of CCDC43 expression in AGS and BGC-823 cell lines. (B, C) DNA synthesis of AGS or BGC-823 cells were measured by EdU incorporation assay after the indicated transfection. **, P < 0.05; ***, P < 0.01. (D, E) The growth of GC cells were examined with colony forming assay. ***, P < 0.01. (F) The GC cell migration assays were performed. *, P > 0.05; **, P < 0.05; ***, P < 0.01. (G) Transwell assays were performed to determine the invasion capacity of control and transfected GC cells. ***, P < 0.01, 100 μm in (B).