Figure 5. Protein expression level of HMGA1, SUZ12 and CCDC43 in human GC patients. (A) SUZ12-related protein-protein interaction (PPI) network from the STRING database. (B) Expression of HMGA1, SUZ12 and CCDC43 protein in each of the primary GC (T) and adjacent noncancerous tissues (N) paired from the same patient by western blotting. The protein expression levels were quantified by comparing the gray level of each band using Quantity One Software (below). (C) Immunohistochemical staining of HMGA1, SUZ12 and CCDC43 in gastric tissues. (D) The correlation between SUZ12 and HMGA1, between CCDC43 and HMGA1, or between SUZ12 and CCDC43 in GC tissues. (E, F) Immunohistochemical analysis of three protein expression in metastatic lymph nodes. Scale bars, 50 μm in (C, F).