Figure 1. Levobupivacaine inhibits proliferation and promotes apoptosis of NSCLC cells. (A–E) The A549 and A427 cells were treated with levobupivacaine (2 mM) or equal volume saline. (A and B) The cell viability was analyzed by the MTT assays in the cells. (C and D) The cell proliferation was measured by the colony formation assays in the cells. (E and F) The cell apoptosis was measured by flow cytometry analysis in the cells. (G and H) The cell cycle was analyzed by flow cytometry analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01.