Figure 2. A representative of C3H mature DC and mixed lymphocyte reactions. (A) The C3H DC cultured in GM-CSF and IL-4 expressed mature phenotype with high levels of CD40, CD80, CD86 and I-Ak. (B) When this mature C3H DC was applied to activate enriched T-cells derived from aged or young B10 mice, MLR showed that proliferation capacity of T-cells from aged mice was lower than that of T-cells from young mice (0.350 ± 0.003 O.D. versus 0.430 ± 0.017 O.D. at responders/stimulatory cells = 100/1, p = 0.001).