Research Paper Volume 13, Issue 12 pp 16229—16247

Testosterone ameliorates age-related brain mitochondrial dysfunction

TP supplementation increases mitochondrial content in the SN and HIPP of aged male rats. (A) Citrate synthase activity in the SN. (B) mtDNA copy number in the SN. (C–E) mRNA levels of ND1, COX1, and ATP6 in the SN. (F) Representative western blots of ND1, COX1, and ATP6 expression in the SN. (G–I) Quantification of ND1, COX1, and ATP6 protein levels in the SN (normalized to β-actin). (J) Citrate synthase activity in the HIPP. (K) mtDNA copy number in the HIPP. (L–N) mRNA levels of ND1, COX1, and ATP6 in the HIPP. (O) Representative western blots of ND1, COX1, and ATP6 expression in the HIPP. (P–R) Quantification of ND1, COX1, and ATP6 expression in the HIPP (normalized to β-actin). Data are expressed as the mean ± S.D. (n = 5/group). *P **P #P U test, Bonferroni correction).

Figure 10. TP supplementation increases mitochondrial content in the SN and HIPP of aged male rats. (A) Citrate synthase activity in the SN. (B) mtDNA copy number in the SN. (CE) mRNA levels of ND1, COX1, and ATP6 in the SN. (F) Representative western blots of ND1, COX1, and ATP6 expression in the SN. (GI) Quantification of ND1, COX1, and ATP6 protein levels in the SN (normalized to β-actin). (J) Citrate synthase activity in the HIPP. (K) mtDNA copy number in the HIPP. (LN) mRNA levels of ND1, COX1, and ATP6 in the HIPP. (O) Representative western blots of ND1, COX1, and ATP6 expression in the HIPP. (PR) Quantification of ND1, COX1, and ATP6 expression in the HIPP (normalized to β-actin). Data are expressed as the mean ± S.D. (n = 5/group). *P < 0.05, **P < 0.01 (Bonferroni test); #P < 0.0167 (Mann-Whitney U test, Bonferroni correction).