Research Paper Volume 13, Issue 12 pp 16267—16286

Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

LncRNA HCP5 is overexpressed in HCC tissues and cell lines. (A) The expression level of HCP5 in HCC tissues is significantly higher compared to that in paracancerous tissues (***pB) HCP5 is overexpressed in HCC cell lines (***pC) The expression of HCP5 in tumors with a size >5 cm, migrated HCC tissues, recurrent cancer tissues, and high histological grade tumor tissues was significantly higher (**pD) FISH assay indicating that HCP5 was predominantly localized in the cytoplasm of Hep3B cell lines.

Figure 1. LncRNA HCP5 is overexpressed in HCC tissues and cell lines. (A) The expression level of HCP5 in HCC tissues is significantly higher compared to that in paracancerous tissues (***p<0.001, GAPDH used served as the internal control). (B) HCP5 is overexpressed in HCC cell lines (***p<0.001, **p<0.01), and the expression level is low in normal hepatocytes. (C) The expression of HCP5 in tumors with a size >5 cm, migrated HCC tissues, recurrent cancer tissues, and high histological grade tumor tissues was significantly higher (**p<0.01, ***p<0.001). (D) FISH assay indicating that HCP5 was predominantly localized in the cytoplasm of Hep3B cell lines.