Research Paper Volume 13, Issue 12 pp 16267—16286

Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

miR-29b-3p reverses HCC proliferation, migration and invasion that is attenuated by sh-HCP5. (A) The quantities of miR-29b-3p reduce in HCP5 knockdown cells when treated with an miR-29b-3p inhibitor. (B) miR-29b-3p is up-regulated when HCP5 is knocked down, and when cells were treated with miR-29b-3p inhibitor, the proliferation increased both in HCCLM3 and Hep3B cell lines (**pC, D) Down-regulation of miR-29b-3p significantly promotes cell migration and invasion (**pE) Cell apoptosis decreases after administration of miR-29b-3p inhibitor to two cell lines that were transfected with sh-HCP5. (F) miR-29b-3p inhibitor restores the EMT progress induced by sh-HCP5 both in HCCLM3 and Hep3B cell lines.

Figure 6. miR-29b-3p reverses HCC proliferation, migration and invasion that is attenuated by sh-HCP5. (A) The quantities of miR-29b-3p reduce in HCP5 knockdown cells when treated with an miR-29b-3p inhibitor. (B) miR-29b-3p is up-regulated when HCP5 is knocked down, and when cells were treated with miR-29b-3p inhibitor, the proliferation increased both in HCCLM3 and Hep3B cell lines (**p<0.01). (C, D) Down-regulation of miR-29b-3p significantly promotes cell migration and invasion (**p<0.01, ***p<0.001). (E) Cell apoptosis decreases after administration of miR-29b-3p inhibitor to two cell lines that were transfected with sh-HCP5. (F) miR-29b-3p inhibitor restores the EMT progress induced by sh-HCP5 both in HCCLM3 and Hep3B cell lines.