Research Paper Volume 13, Issue 12 pp 16471—16484

LncRNA HCG11/miR-579-3p/MDM2 axis modulates malignant biological properties in pancreatic carcinoma via Notch/Hes1 signaling pathway

Knockdown of HCG11 inhibited the tumor growth in xenograft mouse model. (A) We examined the effect of knockdown of HCG11 on the regulation of subcutaneous transplantation mouse model of pancreatic carcinoma by using AsPC-1 cells. (B) Four weeks later, the tumor weight of sh-HCG11 group and shRNA NC group was measured. (C) qPCR assay was applied to detect HCG11 expression (serum) in sh-HCG11 group and shRNA NC group. (D) HCG11 expression (tissues) in sh-HCG11 group and shRNA NC group was also detected by qPCR assay. (E) The expression level of miR-579-3p (tissues) in sh-HCG11 group and shRNA NC group was measured by qPCR. (F, G) The mRNA and protein levels of MDM2 (tissues) in sh-HCG11 group and shRNA NC group were measured by qPCR and western blotting assays. (H) The levels of proliferation associated proteins including Ki67 and PCNA were detected by western blotting assay in sh-HCG11 group and shRNA NC group. (I) The TUNEL-positive cells in sh-HCG11 group and shRNA NC group were measured by TUNEL assay. (J) Western blotting assay was applied to detect E-cadherin and Vimentin expression in sh-HCG11 group and shRNA NC group. *p**p**p

Figure 6. Knockdown of HCG11 inhibited the tumor growth in xenograft mouse model. (A) We examined the effect of knockdown of HCG11 on the regulation of subcutaneous transplantation mouse model of pancreatic carcinoma by using AsPC-1 cells. (B) Four weeks later, the tumor weight of sh-HCG11 group and shRNA NC group was measured. (C) qPCR assay was applied to detect HCG11 expression (serum) in sh-HCG11 group and shRNA NC group. (D) HCG11 expression (tissues) in sh-HCG11 group and shRNA NC group was also detected by qPCR assay. (E) The expression level of miR-579-3p (tissues) in sh-HCG11 group and shRNA NC group was measured by qPCR. (F, G) The mRNA and protein levels of MDM2 (tissues) in sh-HCG11 group and shRNA NC group were measured by qPCR and western blotting assays. (H) The levels of proliferation associated proteins including Ki67 and PCNA were detected by western blotting assay in sh-HCG11 group and shRNA NC group. (I) The TUNEL-positive cells in sh-HCG11 group and shRNA NC group were measured by TUNEL assay. (J) Western blotting assay was applied to detect E-cadherin and Vimentin expression in sh-HCG11 group and shRNA NC group. *p<0.05, **p<0.01, **p<0.001 vs. shRNA NC.