Research Paper Volume 13, Issue 13 pp 17190—17201

miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis

Inhibitory effect of BMSCs-derived exosomes on osteogenesis. Osteogenic BMSC cells were treated with exosomes from normal bone and osteoporosis patients for 7 days, respectively. (A, B) The miR-424-5p and WIF1 levels were verified by qRT-PCR. (C) Western blot was implemented to examine the protein expression of WIF1/Wnt/β-catenin. (D–F) The expression of OPN, OCN and Runx2 mRNAs were compared by qRT-PCR. (G) Western blot was implemented to examine the protein expression of OPN, OCN and Runx2. (H) ALP staining. ***PP

Figure 2. Inhibitory effect of BMSCs-derived exosomes on osteogenesis. Osteogenic BMSC cells were treated with exosomes from normal bone and osteoporosis patients for 7 days, respectively. (A, B) The miR-424-5p and WIF1 levels were verified by qRT-PCR. (C) Western blot was implemented to examine the protein expression of WIF1/Wnt/β-catenin. (DF) The expression of OPN, OCN and Runx2 mRNAs were compared by qRT-PCR. (G) Western blot was implemented to examine the protein expression of OPN, OCN and Runx2. (H) ALP staining. ***P<0.01, ***P<0.001 (vs. Con(Exo)).