Research Paper Volume 13, Issue 13 pp 17190—17201
miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis
Figure 4. miR-424-5p targeted WIF1. (A) The downstream molecules of miR-424-5p were predicted by StarBase database (https://web.archive.org/web/20110222111721/http://starbase.sysu.edu.cn/), and miR-424-5p was found to be the target of WIF1. (B) The binding association between miR-424-5p and WIF1 was testified by dual-luciferase reporter assay. (C) RIP was conducted to determine the targeting correlation between miR-424-5p and WIF1. (D) IP was applied to examine the association between WIF1 and Wnt. (E, F) MiR-424-5p and WIF1 levels were tested by qRT-PCR. NSP>0.05, **P<0.01, ***P<0.001 (vs. miR-NC, vs. Con(Exo)). &&P<0.01, &&&P<0.001 (vs. Con(Exo)).