Research Paper Volume 13, Issue 13 pp 17190—17201

miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis

BMSCs-derived exosomes inhibited osteogenesis via miR-424-5p/WIF1. The WIF1 overexpressed plasmid was transfected into BMSCs-derived exosomes. (A–C) The expression of OPN, OCN and Runx2 were compared by qRT-PCR. (D) ALP staining was used to examine ALP activity. (E) Western blot was applied to determine the protein expression of OPN, OCN and Runx2. (F) The protein expression of WIF1/Wnt/β-catenin was verified by western blot. **P

Figure 5. BMSCs-derived exosomes inhibited osteogenesis via miR-424-5p/WIF1. The WIF1 overexpressed plasmid was transfected into BMSCs-derived exosomes. (AC) The expression of OPN, OCN and Runx2 were compared by qRT-PCR. (D) ALP staining was used to examine ALP activity. (E) Western blot was applied to determine the protein expression of OPN, OCN and Runx2. (F) The protein expression of WIF1/Wnt/β-catenin was verified by western blot. **P<0.01, ***P<0.001 (vs. Con(Exo)). &&P<0.01, &&&P<0.001 (vs. OP(Exo)+PCDNA-NC).