Research Paper Volume 13, Issue 12 pp 16513—16526

Deoxyribonuclease 1-like 3 may be a potential prognostic biomarker associated with immune infiltration in colon cancer

Expression of DNASE1L3 in colon cancer tissue samples and its effect on proliferation and motor ability of colon cancer cells. (A) The expression of DNASE1L3 in colon cancer and paracancerous tissue samples was detected by RealTime-PCR (2-ΔΔCt was used as the calculation method, normalized with β-actin). (B) Comparison of ΔCt values of colon cancer and paracancerous tissue samples homogenized by internal reference β-actin (paired T test, * P=0.036). (C) The expression of DNASE1L3 in 5 colon cancer cell lines detected by Western blot. (D, E) The cell proliferation ability was detected by CCK8 in SW480 (D) and SW48 cells (E) after transfection with DNASE1L3+ plasmid or NC plasmid (P=0.75, 0.60, respectively). (F) Wound healing assay in vitro was used to evaluate the ability of wound closure in SW480 and SW48 cells after transfection with DNASE1L3+ plasmid or NC plasmid (1h, 24h, 48h photography, ×10).

Figure 9. Expression of DNASE1L3 in colon cancer tissue samples and its effect on proliferation and motor ability of colon cancer cells. (A) The expression of DNASE1L3 in colon cancer and paracancerous tissue samples was detected by RealTime-PCR (2-ΔΔCt was used as the calculation method, normalized with β-actin). (B) Comparison of ΔCt values of colon cancer and paracancerous tissue samples homogenized by internal reference β-actin (paired T test, * P=0.036). (C) The expression of DNASE1L3 in 5 colon cancer cell lines detected by Western blot. (D, E) The cell proliferation ability was detected by CCK8 in SW480 (D) and SW48 cells (E) after transfection with DNASE1L3+ plasmid or NC plasmid (P=0.75, 0.60, respectively). (F) Wound healing assay in vitro was used to evaluate the ability of wound closure in SW480 and SW48 cells after transfection with DNASE1L3+ plasmid or NC plasmid (1h, 24h, 48h photography, ×10).