Figure 8. Experimental verification in colon cells and tissues. (A) qPCR was performed to identify the relative expression of CBLN2 and TMEM220 in NCM460 and SW480 cells. (B) qPCR was carried out to assess CBLN2 and TMEM220 expression levels in SW480 cells before and after treatment with 5-aza. (C) Methylation status of CBLN2 and TMEM220 was determined by MSP in SW480 and SW620 cells. (D) Schematic diagrams of CpG islands in the promoter regions of CBLN2 and TMEM220. (E) Representative images of immunohistochemistry staining of colon sections from colon adenocarcinoma (COAD) tissues (n = 46). Original magnification, ×100. (F) Prognostic significance of CBLN2 and TMEM220 expression in COAD patients. (G) Knockdown of RBM47 gene in sw480 cells.