Research Paper Volume 13, Issue 13 pp 17316—17327

Anoctamin 5 promotes osteosarcoma development by increasing degradation of Nel-like proteins 1 and 2

ANO5 was upregulated in osteosarcoma. (A) A volcano plot showed that the ANO5 gene was significantly upregulated in osteosarcoma cells. (B) qRT-PCR indicated that ANO5 was upregulated in osteosarcoma cells (U2OS, MG63, HOS, and Saos2) compared to the normal osteoblast cell line hFOB 1.19. (C, D) Western blot indicated that ANO5 was upregulated in osteosarcoma cells (U2OS, MG63, HOS, and Saos2) compared to the normal osteoblast cell line hFOB 1.19. (E) IHC demonstrated that ANO5 was more highly expressed in osteosarcoma tissues than in adjacent normal tissues. *, PP

Figure 1. ANO5 was upregulated in osteosarcoma. (A) A volcano plot showed that the ANO5 gene was significantly upregulated in osteosarcoma cells. (B) qRT-PCR indicated that ANO5 was upregulated in osteosarcoma cells (U2OS, MG63, HOS, and Saos2) compared to the normal osteoblast cell line hFOB 1.19. (C, D) Western blot indicated that ANO5 was upregulated in osteosarcoma cells (U2OS, MG63, HOS, and Saos2) compared to the normal osteoblast cell line hFOB 1.19. (E) IHC demonstrated that ANO5 was more highly expressed in osteosarcoma tissues than in adjacent normal tissues. *, P<0.05; **, P<0.01.