Figure 4. ANO5 bound to and promoted degradation of NELL1 and NELL2. (A) The protein-protein interaction network showed that ANO5 bound to NELL1 and NELL2. (B, C) Western blot showed that ANO5 overexpression decreased, while ANO5 knockdown increased, the expression of NELL1 and NELL2. (D) Immunoprecipitation demonstrated that ANO5 directly bound with NELL1 and NELL2. (E) Overexpression of ANO5 increased the degradation of NELL1 and NELL2. (F) ANO5 was colocalized with NELL1 and NELL2 in osteosarcoma cells. *, P<0.05; **, P<0.01.