Research Paper Volume 13, Issue 12 pp 15917—15941

Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization

PDS downregulates Brca1 in primary neurons but not in primary astrocytes and microglial cells. (A) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM PDS overnight (18 h). Neurons were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p=0.05 (t=4.304> t0.975,2 = 4.303; significant). Results were pooled from duplicates of 2–4 independent reactions. (B) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM etoposide overnight (18 h). Cells were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p

Figure 4. PDS downregulates Brca1 in primary neurons but not in primary astrocytes and microglial cells. (A) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM PDS overnight (18 h). Neurons were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p=0.05 (t=4.304> t0.975,2 = 4.303; significant). Results were pooled from duplicates of 2–4 independent reactions. (B) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM etoposide overnight (18 h). Cells were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p<0.038, ****p< 0.0001 (t-test). Results were pooled from duplicates of 2–4 independent reactions.