Figure 1. miR-144-3p expression was significantly downregulated and associated with poor prognosis in CRA. (A) miR-144-3p expression was significantly downregulated in CRATs. The levels of miR-144-3p in 160 paired CRATs and NCMTs from training cohort were determined by qRT-PCR. Data were analyzed using 2-ΔCt method. (B) Expression levels of miR-144-3p in CRATs with advanced TNM stage (TNM III) were significantly lower than in early TNM stage (TNM I/II). (C) miR-144-3p expression level gradually increased from LMNs, CRATs to NCMTs. The expression levels of miR-144-3p were determined and compared in 8 matched NCMTs, CRATs, and LMNs. (D) Data from GSE44121 were used to compare miR-144-3p expression levels in LMNs and CRATs. Overall survival (E) and disease-free survival (F) were compared by Kaplan-Meier method based on miR-144-3p expression in the training cohort. Overall survival (G) and disease-free survival (H) were compared by Kaplan-Meier method based on miR-144-3p expression in the validation cohort. NCMTs, noncancerous colorectal mucosae tissues; CRATs, colorectal adenocarcinoma tissues; LMNs, liver metastatic nodules. *, P<0.05; ***, P < 0.001.