Research Paper Volume 13, Issue 13 pp 17349—17369

miR-144-3p inhibited the growth, metastasis and epithelial-mesenchymal transition of colorectal adenocarcinoma by targeting ZEB1/2

miR-144-3p was downregulated in CRA cell lines and inhibited CRA cell proliferation and growth. (A) miR-144-3p expression was downregulated in CRA cells lines HCT116, Lovo, SW480, SW620, HT-29 compared to normal colorectal mucosal cell line FHC. (B) qRT-PCR was performed to verify the efficiency of overexpression in Lovo cells transfected with miR-144-3p mimic and knockdown in HCT116 cells transfected with miR-144-3p inhibitor. (C) MTT assay of Lovo and HCT116 cell proliferation. (D) Colony formation assay of Lovo and HCT116 cells. (E) Edu assay of the proliferation ability of Lovo cells transfected with miR-144-3p mimic and HCT116 cells transfected with miR-144-3p inhibitor. (F) The growth curve showed the change of the volume of subcutaneous tumors from indicated CRA cells and the representative images of subcutaneous tumors harvested 6 weeks after CRA cell inoculation in the right panel. Scale bar: 1cm. NC, normal control. **, PP

Figure 2. miR-144-3p was downregulated in CRA cell lines and inhibited CRA cell proliferation and growth. (A) miR-144-3p expression was downregulated in CRA cells lines HCT116, Lovo, SW480, SW620, HT-29 compared to normal colorectal mucosal cell line FHC. (B) qRT-PCR was performed to verify the efficiency of overexpression in Lovo cells transfected with miR-144-3p mimic and knockdown in HCT116 cells transfected with miR-144-3p inhibitor. (C) MTT assay of Lovo and HCT116 cell proliferation. (D) Colony formation assay of Lovo and HCT116 cells. (E) Edu assay of the proliferation ability of Lovo cells transfected with miR-144-3p mimic and HCT116 cells transfected with miR-144-3p inhibitor. (F) The growth curve showed the change of the volume of subcutaneous tumors from indicated CRA cells and the representative images of subcutaneous tumors harvested 6 weeks after CRA cell inoculation in the right panel. Scale bar: 1cm. NC, normal control. **, P<0.01; ***, P < 0.001.