Figure 2. Cytotoxic effects of combination treatment with RAPA or/and SAHA in irradiated NSCLC cells. Survival fractions were assessed by the colony formation assay. (A, B) The radiation dose-response survival curves after 24h of treatment with RAPA (100nmol/L) or/and SAHA (2.5μmol/L) in two NSCLC cells A549 (A), SK-MES-1 (B). (C, D) Colony formation assay (C) and flow cytometry analysis of the cell cycle (D) in two NSCLC cells (A549, SK-MES-1) resulting from IR (4Gy) after treatment with RAPA (100nmol/L) or/and SAHA (2.5μmol/L) for 24h.