Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

CircRNA FUT10 sponges miR-365a-3p. (A) starBase software was used to predict miR-365a-3p binding sites on circRNA FUT10. Dual-luciferase reporter assays validated the miR-365a-3p binding sites on circRNA FUT10 in (B) adult and (C) aged SkMSCs. (D) RNA pull-down assay results indicated that miR-365a-3p binding to circRNA FUT10 probes was enriched. (E) miR-365a-3p was analyzed by qPCR. Each bar represents the mean ± SEM. *P

Figure 3. CircRNA FUT10 sponges miR-365a-3p. (A) starBase software was used to predict miR-365a-3p binding sites on circRNA FUT10. Dual-luciferase reporter assays validated the miR-365a-3p binding sites on circRNA FUT10 in (B) adult and (C) aged SkMSCs. (D) RNA pull-down assay results indicated that miR-365a-3p binding to circRNA FUT10 probes was enriched. (E) miR-365a-3p was analyzed by qPCR. Each bar represents the mean ± SEM. *P < 0.05. All experiments were performed at least three times with duplication within each individual experiment.