Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

CircRNA FUT10 suppresses SkMSC proliferation and differentiation by targeting miR-365a-3p. (A) circRNA FUT10 overexpression vectors, miR-365a-3p mimics, and circRNA FUT10 + miR-365a-3p were transfected into SkMSCs. miR-365a-3p expression was examined by real-time qPCR. (B) EdU assay results indicated that upregulated circRNA FUT10 suppressed SkMSC proliferation by regulating miR-365a-3p. (C) Quantification of EdU-positive cells. (D) MyHC and MyoD mRNA were examined by real-time qPCR. (E) Western blot analysis showing MyHC and MyoD protein expression in different groups. (F) Quantification of MyHC and MyoD were normalized to β-actin. Each bar represents the mean ± SEM. *P

Figure 4. CircRNA FUT10 suppresses SkMSC proliferation and differentiation by targeting miR-365a-3p. (A) circRNA FUT10 overexpression vectors, miR-365a-3p mimics, and circRNA FUT10 + miR-365a-3p were transfected into SkMSCs. miR-365a-3p expression was examined by real-time qPCR. (B) EdU assay results indicated that upregulated circRNA FUT10 suppressed SkMSC proliferation by regulating miR-365a-3p. (C) Quantification of EdU-positive cells. (D) MyHC and MyoD mRNA were examined by real-time qPCR. (E) Western blot analysis showing MyHC and MyoD protein expression in different groups. (F) Quantification of MyHC and MyoD were normalized to β-actin. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication within each individual experiment.