Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

Effect of HOXA9 on proliferation and differentiation of SkMSCs (A) qPCR and (B) western blotting were performed to identify HOXA9 mRNA and protein expression in adult and aged SkMSCs. (C) HOXA9 was normalized to β-actin in adult and aged SkMSCs. (D) qPCR and (E) western blotting were performed to identify HOXA9 mRNA and protein expression in adult SkMSCs transfected with circRNA FUT10 overexpression vector, while aged SkMSCs were transfected with circRNA FUT10 small interfering RNA to knock down circRNA FUT10. (F) HOXA9 was normalized to β-actin in the above groups. (G) MTT assay showing the effect of HOXA9 on adult and aged SkMSC proliferation. (H) Western blotting was performed to identify MyHC and MyoD expression in transfected cells. (I) Quantification of HOXA9 was normalized to β-actin. Each bar represents the mean ± SEM. *P

Figure 6. Effect of HOXA9 on proliferation and differentiation of SkMSCs (A) qPCR and (B) western blotting were performed to identify HOXA9 mRNA and protein expression in adult and aged SkMSCs. (C) HOXA9 was normalized to β-actin in adult and aged SkMSCs. (D) qPCR and (E) western blotting were performed to identify HOXA9 mRNA and protein expression in adult SkMSCs transfected with circRNA FUT10 overexpression vector, while aged SkMSCs were transfected with circRNA FUT10 small interfering RNA to knock down circRNA FUT10. (F) HOXA9 was normalized to β-actin in the above groups. (G) MTT assay showing the effect of HOXA9 on adult and aged SkMSC proliferation. (H) Western blotting was performed to identify MyHC and MyoD expression in transfected cells. (I) Quantification of HOXA9 was normalized to β-actin. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication within each individual experiment.