Research Paper Volume 13, Issue 13 pp 17489—17498

HDAC inhibitor Trichostatin A suppresses adipogenesis in 3T3-L1 preadipocytes

TSA suppresses the expression of adipogenic and lipogenic transcriptional factors in 3T3-L1 cells. Cells were incubated with a differentiation cocktail (DMI) medium with TSA (0.5 and 1 μM). (A) mRNA of PPAR-γ; (B) mRNA of C/EBPα (n=5-6, ####, P

Figure 5. TSA suppresses the expression of adipogenic and lipogenic transcriptional factors in 3T3-L1 cells. Cells were incubated with a differentiation cocktail (DMI) medium with TSA (0.5 and 1 μM). (A) mRNA of PPAR-γ; (B) mRNA of C/EBPα (n=5-6, ####, P<0.0001 vs. vehicle group; &&, &&&, P<0.01, 0.001 vs. DMI treatment group).